In 2007, the Human Fertilisation and Embryology Authority in London, UK, published Hybrids and Chimeras: A Report on the Findings of the Consultation, which summarized a public debate about research on, and suggested policy for, human animal chimeras. The HFEA formulated the report after conducting a series of surveys and debates from earlier in 2007. The HFEA issued a statement in September 2007, followed by an official report published on 1 October 2007. Their report on human-animal chimeras set a worldwide precedent for discussions of the ethical use of those embryos in labs. The HFEA's report led the UK government to pass legislature about the use of human-animal cytoplasmic hybrid embryos for research in the UK.

This study aims to provide information to answer the following question: While some scientists claim they can indefinitely culture a stem cell line in vitro, what are the consequences of those culturing practices? An analysis of a cluster of articles from the Embryo Project Encyclopedia provides information to suggest possible solutions to some potential problems in cell culturing, recognition of benefits for existing or historical culturing practices, and identification of gaps in scientific knowledge that warrant further research.

Friedrich Leopold August Weismann published Das Keimplasma: eine Theorie der Vererbung (The Germ-Plasm: a Theory of Heredity, hereafter The Germ-Plasm) while working at the University of Freiburg in Freiburg, Germany in 1892. William N. Parker, a professor in the University College of South Wales and Monmouthshire in Cardiff, UK, translated The Germ-Plasm into English in 1893. In The Germ-Plasm, Weismann proposed a theory of heredity based on the concept of the germ plasm, a substance in the germ cell that carries hereditary information. The Germ-Plasm compiled Weismann's theoretical work and analyses of other biologists' experimental work in the 1880s, and it provided a framework to study development, evolution and heredity. Weismann anticipated that the germ-plasm theory would enable researchers to investigate the functions and material of hereditary substances.

Conrad Hal Waddington's Organisers and Genes, published in 1940, is a summary of available research and theoretical framework for many concepts related to tissue differentiation in the developing embryo. The book is composed of two main conceptual sections. The first section explores the action and nature of the organizer, while the second section delves into genes and their influence on development.

In 1953, Virginia Apgar published the article "A Proposal for a New Method for Evaluation of the Newborn Infant" about her method for scoring newborn infants directly after birth to assess their health and whether medical intervention was necessary. Apgar worked at the Presbyterian Hospital in New York City, New York, as an obstetrical anesthesiologist, a physician who administers pain medication during childbirth. In that capacity, she sought to reestablish clear scoring guidelines for newborn infants so that she could compare which obstetric practices, pain relief methods, and resuscitation methods worked the best during and after childbirth. She published her article in Current Researches in Anesthesia and Analgesia in 1953, and the Apgar scoring system is still used in hospitals around the world as of 2016. In the article, Apgar establishes a scoring system for newborn infants that allows for quick assessment of their health directly after birth and therefore swift intervention by medical personnel to promote healthy development.

In 'Altruism and the Origin of the Worker Caste,' Bert Hölldobler and Edward Osborne Wilson explore the evolutionary origins of worker ants. 'Altruism and the Origin of the Worker Caste' is the fourth chapter of Hölldobler and Wilson's book, The Ants, which was published by The Belknap Press of Harvard University in Cambridge, Massachusetts, in 1990. In 'Altruism and the Origin of the Worker Caste,' Hölldobler and Wilson evaluate various explanations for how a non-reproductive caste of ant evolved. Their investigation into the evolutionary origins of worker ants synthesized research on the reproductive practices of ants to provide an analysis of how sterile groups of organisms persist in a population.

In 2001, Yale University Press published Frederic Lawrence Holmes' book, Meselson, Stahl, and the Replication of DNA: A History of "The Most Beautiful Experiment in Biology" (Replication of DNA), which chronicles the 1950s debate about how DNA replicates. That experiment verified that DNA replicates semi-conservatively as originally proposed by Watson and Crick. Rather than focusing solely on experiments and findings, Holmes's book presents the investigative processes of scientists studying DNA replication. Based on personal accounts, letter correspondence, and preserved research documents, Replication of DNA serves as a detailed account of the initial issues surrounding DNA replication and the Meselson-Stahl experiment from a scientist's perspective.

In 2005, Ernest McCulloch and James Till published the article “Perspectives on the Properties of Stem Cells,” which discusses the various properties and future possibilities for the use of stem cells. Stem cells are unspecialized cells that can develop into several different cell types. In the article published in the journal Nature on 1 October 2005, the authors say they wrote the article to dispel misconceptions about what stem cells are, what they do, address some controversies surrounding stem cells, and discuss potential uses of stem cells. In the article, McCulloch and Till reveal how stem cell research has revolutionized cancer treatment as well as set the stage for future embryonic and adult stem cell research.

In 2011, Sarah McMahon and colleagues published “The Impact of Emotional and Physical Violence During Pregnancy on Maternal and Child Health at One Year Post-partum,” hereafter, “The Impact,” in the journal, Child and Youth Services Review. While existing studies had indicated negative chronic effects resulting from intimate partner violence, or IPV, such as miscarriage and premature labor, there was little research specifically analyzing the separate and joint effects of psychological and physical abuse on pregnant women and fetuses. The authors reported that both physical and emotional IPV had negative impacts on the woman and child at one-year after birth, including worse overall health and increased likelihood of depression. In “The Impact,” the researchers analyzed the effects of partner abuse during pregnancy, distinguishing between the effects of emotional abuse and physical abuse on health outcomes for a pregnant woman and her offspring.

In 1999, researcher Radim Srám, sometimes spelled Radim Šrám, published his article “Impact of Air Pollution on Reproductive Health” in the journal Environmental Health Perspectives. In the article, Srám analyzes the effects of exposure to air pollution, which can include harmful chemicals, on fetal growth and development. Srám discusses how industrialized countries such as the US and China have led to an increase in the global amount of respirable air pollutants. He mentions the influence that air pollution may have on the development of several birth defects, as well as the death of fetuses in the womb and infants after birth. Throughout his article, Srám summarizes the findings of several studies, and he describes the harmful developmental effects of common air pollutants, which has prompted further research in developed and developing countries into how air pollutants can cause birth defects and other diseases.

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