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August Antonius Rauber was an embryologist and anatomist who examined gastrulation in avian embryos. He examined the formation of the blastopore, epiblast, and primitive streak during chick development. Subsequent researchers have further studied Rauber's findings, which has led to new discoveries in embryology and developmental biology.
Twentieth-century researcher Ernest John Christopher Polge studied the reproductive processes of livestock and determined a method to successfully freeze, thaw, and utilize viable sperm cells to produce offspring in animals. In 1949, Polge identified glycerol as a cryoprotectant, or a medium that enables cells to freeze without damaging their cellular components or functions. Several years later, Polge used glycerol in a freezing process called vitrification, which enabled him to freeze poultry sperm, thaw that sperm, and use it to fertilize vertebrate embryos.