Studies in Spermatogenesis (1905), by Nettie Maria Stevens
Keywords: chromosomal sex determination
Studies in Spermatogenesis is a two volume book written by Nettie Maria Stevens, and published by the Carnegie Institution of Washington in 1905 and 1906. In the books Stevens explains the research she conducted on chromosomal sex determination in the sperm and egg cells of insect species while at Bryn Mawr College, near Philadelphia, Pennsylvania. Studies in Spermatogenesis described early examples of chromosomal XY sex-determination.
Studies in Spermatogenesis described research on the generation of sperm cells (spermatogenesis) and their role in the fertilization of egg cells. Like much of the work conducted in genetics at the beginning of the 1900s, Stevens's research was influenced by two events at the turn of the twentieth century. The first was the rediscovery of Gregor Mendel's theories of inheritance in the late 1890s that revitalized the field of genetics. Mendel, who worked in Europe in the mid nineteenth century, developed a law of heredity that provided a framework to explain how a parent could transfer traits to its offspring, even though researchers couldn't describe the the genetic materials that carried these traits. From 1902 to 1903 Walter Sutton at Columbia University in New York, New York and Theodor Boveri in Würzburg, Germany independently worked to identify this unknown genetic material. Sutton and Boveri concluded that chromosomes, the rod-shaped structures located inside the nucleus of every cell, are the genetic material of inheritance, providing a matter for Mendel's Laws.
Prior to Stevens's work, two theories emerged to explain sex determination. The first attributed the effect to environmental conditions, which according to some researchers caused the developmental fates of the embryo. The second ascribed sex determination to the internal factors of organisms, such as the cytoplasm or nucleus of the egg. Stevens's research was part of the internalist approach, and she focused on chromosomal differences in sperm cells. Stevens's research on the chromosome contained in sperm cells stemmed from research on accessory chromosomes conducted a few years earlier by Clarence Erwin McClung, a former teacher of Sutton, at the University of Kansas in Lawrence, Kansas.
In 1902 McClung published "The Accessory Chromosome–Sex Determinant?" In this article, McClung theorized that an odd number of chromosomes found in insects from some species formed a basis of sexual determination by which an extra chromosome caused cells to differentiate differently than normal. McClung's hypothesis influenced Stevens’s work as each insect she studied for Studies in Spermatogenesis was observed to either verify or refute the presence of the extra chromosome. Although Stevens did not find extra chromosomes in her work with aphids in 1904, Stevens held that the sperm and the eggs somehow determined the sex of an offspring.
Studies in Spermatogenesis has two parts. Part I, published in 1905, begins with a reference to McClung's theory of accessory chromosomes and states the need to further investigate the theory as a potential sex determination mechanism. Stevens next introduces five insects previously unstudied for sex determination: termite (Zootermopsis angusticollis), sand cricket (Stenopelmatus), German cockroach (Blattella germanica), mealworm (Tenebrio molitor), and aphid (Aphis oenotherae). Stevens describes the methods used to microscopically view the testes of each insect. Stevens details her results for the individual stages of spermatogenesis and for the presence or absence of accessory chromosomes for each insect. The results also reference figures that are present at the end of the book, providing visual representations of the results. Stevens concludes each section with a summary of the important observations made for each particular insect. Part I concludes with a general discussion of each insect in relation to McClung's hypothesis and reiterates the importance of the findings in Tenebrio molitor, namely that in both somatic and sex cells, one particular chromosome was smaller in male cells than the corresponding chromosome in female cells.
In Part II of Studies in Spermatogenesis, published in 1906, Stevens further discusses her studies on XY sex determination. Stevens focuses on beetles, specifically the species Tenebrio molitor. Stevens searched for similar sexual differentiation mechanisms in species similar to T. molitor to confirm sex determination by a distinct chromosomal element. She identifies nineteen species belonging to eight different families in which sex determination is identified by a small chromosomal element similar to T. molitor.
Stevens devotes a much of Studies in Spermatogenesis to her work with the mealworm by describing both the germ cells (precursor cells to egg and sperm cells) and the somatic cells of mealworms. At the time, researchers had noted that somatic cells contained a number of chromosomes, with one half contributed by each parent. They had also noted that germ cells contained half of the number chromosomes found in somatic cells. In mealworms, Stevens found ten large chromosomes in eggs and either ten large or nine large and one small chromosome in the spermatocytes, which later mature into sperm cells. Stevens also describes the somatic cells of mealworms as containing twenty large chromosomes in females, and nineteen large and one small chromosome in males. After repeatedly observing that the comparatively small chromosomes were present only in male somatic and germ cells, Stevens concluded that an egg fertilized by a male sex cell (spermatozoon) that contains the small chromosome will develop into a male. Likewise, an egg fertilized by a spermatozoon containing the larger chromosome will develop into a female.
Studies in Spermatogenesis influenced biologists such as Edmund Beecher Wilson and Thomas Hunt Morgan, two of Stevens's mentors at Bryn Mawr College. In 1905, Wilson, a professor of Zoology at Columbia University, published "Studies on Chromosomes" in which he describes a form of XY and X0 sex determination. However, Wilson remained skeptical about attributing sex to a particular type of chromosome until further information supported the theory. Wilson suggested instead that the intensity of chromosomes varied between sexes and that environmental causes could affect their intensity. After Stevens published her results in September 1905, Wilson revised his previous publications, deleting references to environmental influences. Instead, Wilson cited Stevens's work as supporting his original theories.
Studies in Spermatogenesis provides one of the first observations of XY sex-determination. Stevens's findings allowed researchers to locate the material of Mendelian inheritance that passed specific traits, in this case the sex of the organism Tenebrio molitor, through a distinct chromosomal element.
- Boveri, Theodor. "Über mehrpolige Mitosen als Mittel zur Analyse des Zellkerns." [On Multipolar Mitosis as a Means of Analysis of the Cell Nucleus]. Physikalisch-Medizinischen Gesellschaft zu Würzburg 35 (1902): 67–90. http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/15991 (Accessed December 16, 2013)
- Brush, Stephen G. "Nettie M. Stevens and the Discovery of Sex Determination by Chromosomes." Isis 69 (1978): 162–72.
- Lenay, Charles. "Hugo De Vries: From the Theory of Intracellular Pangenesis to the Rediscovery of Mendel." Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie 323 (2000): 1053–60.
- Maienschein, Jane. "What Determines Sex? A Study of Converging Approaches, 1880–1916." Isis 75 (1984): 457–80.
- McClung, Clarence Erwin. "The Accessory Chromosome–Sex Determinant?" The Biological Bulletin 3 (1902): 43–84. http://www.biolbull.org/content/3/1-2/43.full.pdf+html (Accessed April 25, 2013).
- Mendel, Gregor Johann. “Versuche über Pflanzen-Hybriden” [Experiments Concerning Plant Hybrids].” Verhandlungen des naturforschenden Vereines in Brünn [Proceedings of the Natural History Society of Brünn] IV (1865): 3–47. Reprinted in Fundamenta Genetica, ed. Jaroslav Kříženecký, 15–56. Prague: Czech Academy of Sciences, 1966. http://www.mendelweb.org/Mendel.html (Accessed January 17, 2014).
- Ogilvie, Marilyn Bailey and Clifford J. Choquette. "Nettie Maria Stevens (1861-1912): Her Life and Contributions to Cytogenetics." Proceedings of the American Philosophical Society 125 (1981): 292–311.
- Stevens, Nettie Maria. Studies in Spermatogenesis with Especial Reference to the "Accessory Chromosome." Washington D.C.: Carnegie Institution of Washington, 1905. http://www.archive.org/details/studiesinspermat01stevrich (Accessed April 25, 2013).
- Stevens, Nettie Maria. Studies in Spermatogenesis with a Comparative Study of the Heterochromosomes in Certain Species of Coleoptera, Hemiptera and Lepidoptera, with Especial Reference to Sex Determination. Washington D. C.: Carnegie Institution of Washington, 1906. http://www.archive.org/details/studiesinspermat01stevrich (Accessed April 25, 2013).
- Sutton, Walter S. "On the morphology of the chromosome group in Brachystola magna." The Biological Bulletin 4 (1902): 24–39. http://www.biolbull.org/content/4/1/24.full.pdf+html (Accessed April 25, 2013).
- Wilson, Edmund Beecher. “Studies on Chromosomes,” 1–13 in Journal of Experimental Zoology, (1905–1912). http://dx.doi.org/10.5962/bhl.title.23656 (Accessed January 17, 2014).